WesternBlots -
ImmunoBlots
Table of detectable auto-antibodies
|
Type of Antibody |
Molecular Weight, (kD) |
Specificity of the antigens |
|
anti-AMA (M2-Type) Mitochondrial antibodies are directed against proteins of the inner and outer membrane of the mitochondria and react with proteins of the a cetoacid dehydrogenase complex of the mitochondria |
74 kD 50 kD, 54 kD Other additional bands at 54 kD and 52 kD are rather close to the LP- and LKM antigens. |
Highly specific and highly sensitive marker for primary biliary cirrhosis (PBC), a chronic cholestatic liver disease. In M2-positive samples the major band against the 74 kD Antigen must be present!! |
|
anti-LKM The so-called `liver-kidney-microsomal` antibodies (anti-LKM) are directed against proteins of the cytochrom-P-450 system |
50 kD |
They are a marker for autoimmune chronic active hepatitis. These antibodies are specific for a subgroup of the autoimmune hepatitis and are mainly detected in younger patients, aging from childhood up to less than 30 years of age. |
|
anti-LP These antibodies are directed against a cytoplasmatic antigen |
51 kD |
Disease-specific anti-LP (liver-pancreas / anti-SLA) antibodies are associated with chronic active hepatitis and are found in approx. 25 percent of CAH-patients. |
In patients with autoimmune disease anticytoplasmatic antibodies are found, whereas in cases of lupoid hepatitis also anti-nuclear antibodies may occur.