WesternBlots - ImmunoBlots                             

 

Treponema pallidum IgG + IgM Immunoblot

 

Immunoblot for the detection of IgG or IgM antibodies against Treponema pallidium in serum

 SUMMARY AND EXPLANATION OF THE TEST

The species Treponema includes the human pathogenic kind:

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Treponema pallidum ssp. pallidum (venereal syphilis)

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Treponema pallidum ssp. endemicum (endemic syphilis)

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Treponema pallidum ssp. pertenue (frambesia, yaws)

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Treponema carateum (Pinta)

Other Treponema including nonpathogenic species appears in the human oral cavity, in the intestinal tract and in the genital tract. Genital treponema with low pathogenity appears often together with anaerobic gram-negative bacillus in genital ulcera (so called fusospirillary infections). They cannot be separated in the darkfield from Treponema pallidum.

Treponema pallidum is spread worldwide. There are no climatical, racial or geographical barriers for syphillis. The only known host is man. In adults, it is almost always sexually transmitted. Through a syphillitic mother, Treponema pallidum can be transferred diaplacentally at any time during the fetal period.

EVALUATION AND INTERPRETATION OF THE RESUTS

This test makes a proof of specific antibodies against Treponema pallidum in serum possible.

Cutoff - Standard setting: IgM/ IgA - 10 %, IgG -  20 %

Proofable are antibodies against antigens with a molecular weight of:

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15.5 kD

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17 kD

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45.5 kD

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47 kD

Interpretation IgG

Positive reaction - if 2 bands (17kD and 47 kD) can be definitely detected.

Possibly positive reaction - if 1 of the 2 bands 17 kD and 47 kD can be detected or if 2 bands (15,5 kD and 45,5 kD) are detected.

Interpretation IgM

Positive reaction - if only 1 of the 4 bands (15.5 kD, 17 kD, 45.5 kD or 47 kD) is detected.

Finally, it should be mentioned that every single result, also a blot result, must always be looked at in the context with further data. A final diagnosis whether there is an infection or not can only be made after consideration of all laboratory data and information from the clinic.

 

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